Journal: Acta biomaterialia
Article Title: 3D printed elastomeric biomaterial mitigates compaction during in vitro vasculogenesis
doi: 10.1016/j.actbio.2023.09.026
Figure Lengend Snippet: Imaging of temporal progression of pre-vascular networks. Macro (A & C; scalebar = 2 mm) and micro (B & D; scalebar = 100 μm) scale morphology of GFP-endothelial cells (red) in free fibrin hydrogel (A-B) or vascular-3D printed scaffolds (V-3DPSS; C-D) on days 3 (i), 5 (ii), and 7 (iii). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Article Snippet: Single donor, human umbilical vascular endothelial cells (HU-VECs; Passage 1–6; Cat. No. C2517A; Lonza) or green fluorescent protein-expressing HUVECs (GFP-HUVEC; Passage 1–6; Cat. No. cAP-0001GFP; Angio-Proteomie) were cultured as monolayers in tissue culture treated flasks with media changed every 2 days.
Techniques: Imaging